The researchers screened for molecules that could, in combination, reverse cellular aging and rejuvenate human cells. They developed high-throughput cell-based assays to distinguish young cells from old and senescent cells, including transcription-based aging clocks and a real-time nucleocytoplasmic protein compartmentalization (NCC) assay. In an exciting discovery, the team identified six chemical cocktails that restore NCC and genome-wide transcript profiles to youthful states.
Identification of Chemical Cocktails for Cellular Rejuvenation
The researchers used a combination of approaches to identify chemical cocktails that could reverse cellular aging. They screened for molecules that could restore NCC and transcriptional profiles to a youthful state. The team developed high-throughput assays to distinguish between young, old, and senescent cells, including transcription-based aging clocks and the NCC assay.
After extensive screening, the researchers identified six chemical cocktails that were able to reverse cellular aging and rejuvenate human cells.